What Does the “Love Hormone” Do? It’s Complicated: Weizmann Institute of Science researchers

The findings could shed new light on efforts to use oxytocin to treat variety of psychiatric conditions, from social anxiety, autism to schizophrenia

During the pandemic lockdown, as couples have been forced to spend days and weeks in one another’s company, some have found their love renewed while others are on their way to divorce court. Oxytocin, a peptide produced in the brain, is complicated in that way: a neuromodulator, it may bring hearts together or it can help induce aggression. That conclusion arises from unique research led by Weizmann Institute of Science researchers in which mice living in semi-natural conditions had their oxytocin-producing brain cells manipulated in a highly precise manner. The findings, which were published in Neuron, could shed new light on efforts to use oxytocin to treat a variety of psychiatric conditions, from social anxiety and autism to schizophrenia.

Much of what we know about the actions of neuromodulators like oxytocin comes from behavioural studies of lab animals in standard lab conditions. These conditions are strictly controlled and artificial, in part so that researchers can limit the number of variables affecting behaviour. But a number of recent studies suggest that the actions of a mouse in a semi-natural environment can teach us much more about natural behaviour, especially when we mean to apply those findings to humans.

Prof Alon Chen’s lab group in the Institute’s Neurobiology Department have created an experimental setup that enables them to observe mice in something approaching their natural living conditions – an environment enriched with stimuli they can explore – and their activity is monitored day and night with cameras and analysed computationally. The study, which has been ongoing for the past eight years, was led by research students Sergey Anpilov and Noa Eren, and Staff Scientist Dr Yair Shemesh in Prof Chen’s lab at the Weizmann Institute of Science. The innovation in this experiment, however, was to incorporate optogenetics – a method that enables researchers to turn specific neurons in the brain on or off using light. To create an optogenetic setup that would enable the team to study mice that were behaving naturally, the group developed a compact, lightweight, wireless device with which the scientists could activate nerve cells by remote control. With the help of optogenetics expert Prof Ofer Yizhar of the same department, the group introduced a protein previously developed by Prof Yizhar into the oxytocin-producing brain cells in the mice. When light from the wireless device touched those neurons, they became more sensitised to input from the other brain cells in their network.

“Our first goal,” says Anpilov, “was to reach that ‘sweet spot’ of experimental setups in which we track behaviour in a natural environment, without relinquishing the ability to ask pointed scientific questions about brain functions.”

Dr Shemesh adds that, “The classical experimental setup is not only lacking in stimuli, the measurements tend to span mere minutes, while we had the capacity to track social dynamics in a group over the course of days.”

Delving into the role of oxytocin was sort of a test drive for the experimental system. It had been believed that this hormone mediates pro-social behaviour. But findings have been conflicting, and some have proposed another hypothesis, termed ‘social salience’ stating that oxytocin might be involved in amplifying the perception of diverse social cues, which could then result in pro-social or antagonistic behaviours, depending on such factors as individual character and their environment.

To test the social salience hypothesis, the team used mice in which they could gently activate the oxytocin-producing cells in the hypothalamus, placing them first in the enriched, semi-natural lab environments. To compare, they repeated the experiment with mice placed in the standard, sterile lab setups.

In the semi-natural environment, the mice at first displayed heightened interest in one another, but this was soon accompanied by a rise in aggressive behaviour. In contrast, increasing oxytocin production in the mice in classical lab conditions resulted in reduced aggression. “In an all-male, natural social setting, we would expect to see belligerent behaviour as they compete for territory or food,” says Anpilov. “That is, the social conditions are conducive to competition and aggression. In the standard lab setup, a different social situation leads to a different effect for the oxytocin.”

If the ‘love hormone’ is more likely a ‘social hormone’, what does that mean for its pharmaceutical applications? “Oxytocin is involved, as previous experiments have shown, in such social behaviours as making eye contact or feelings of closeness,” says Eren, “but our work shows it does not improve sociability across the board. Its effects depend on both context and personality.” This implies that if oxytocin is to be used therapeutically, a much more nuanced view is needed in research: “If we want to understand the complexities of behaviour, we need to study behaviour in a complex environment. Only then can we begin to translate our findings to human behaviour,” she says.

Participating in this research were scientists at the Max Planck Institute for Psychiatry in Munich, including research students Asaf Benjamin and Stoyo Karamihalev, staff scientist Dr Julien Dine and postdoctoral fellow Dr Oren Forkosh of the Chen lab; Prof Shlomo Wagner and postdoctoral fellow Dr Hala Harony-Nicolas of Haifa University; Prof Inga Neumann and research student Vinicius Oliveira of Regensburg University, Germany; and electrical engineer Avi Dagan.

Prof Chen’s research is supported by the Ruhman Family Laboratory for Research in the Neurobiology of Stress; the Perlman Family Foundation, Founded by Louis L and Anita M Perlman; the Fondation Adelis; Bruno Licht; and Sonia T Marschak. Prof Chen is the incumbent of the Vera and John Schwartz Professorial Chair in Neurobiology.

Prof Yizhar’s research is supported by the Ilse Katz Institute for Material Sciences and Magnetic Resonance Research; the Adelis Brain Research Award; and Paul and Lucie Schwartz, Georges and Vera Gersen Laboratory.

AutismLove HormoneoxytocinProf Alon ChenProf Ofer Yizharpsychiatric conditionsschizophreniasocial anxietyWeizmann Institute of Science
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