Dr Rajesh Rengarajan, Product Manager-Clinical Chemistry, DiaSys Diagnostic India, gives an insight on the technique of immunoturbidimetry
Immunoturbidimetry (IT) is an important technique in the broad diagnostic field of clinical chemistry. It is used to determine serum proteins which are not detectable with classical clinical chemistry methods and antigen-antibody reaction. Immunoturbidimetry is based on specific and sensitive reactions between an antigen and an antibody. Each antibody binds to a specific antigen (key and lock).
Antigens are substances that induce an immune response (production of antibodies). The immunoturbidimetry is further classified into two types such as direct immunoturbidimetry and particle enhanced turbidimetric immuno assay (PETIA).The principle of precipitation reaction is the interaction of antigen with antibody leading to the production of antigen-antibody complexes. The Prozone security is an optimal antibody-antigen ratio that leads to maximal precipitation. If the antigen concentration exceeds a certain level, antibody saturation occurs, followed by decreased precipitation and, in turn, lowers measuring signals. This effect is described as high dose hook orantigen excess effect. The advantages of immunoturbidimetry versus the conventional slide agglutination method are the high sensitivity, quantitative result, high prozone security, and independency of manual application. The disadvantages of agglutination slide tests are the differing drop size of sample or latex reagent , the contamination of reagent or use by splattering, visual interpretation (depends on user), time-consuming and labor intense, qualitative (+ or -) or semi-quantitative results (+,++,+++,++++), and false differentiation of borderline cases.
DiaSys immunoturbidimetric tests are provided in various kits for multi-purpose or automated use on common clinical chemistry analysers. DiaSys offers a broad range of human matrix based calibrators and controls for general clinical chemistry, lipid parameters, specific proteins, etc. with a variety of interesting analytes. DiaSys immunoturbidimetry has a wide measuring range in combination with high prozone security, no sample dilution and reliable results for all normal and pathological samples due to high prozone limit. It also provides extremely sensitive results (LOD as low as zeptomole level), due to the absence of interfering emissions (i.e. high specificity) leaving No Doubt in immunoturbidimetry test.
Contact Details:
DiaSys Diagnostics India
Plot# 821, TTC Indl. Area,
Mahape, MIDC,
Navi Mumbai – 400 710
Mob: +91 97668 32134
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